Nanometer Scale Cell Surface Topography for Label-Free Cell Classification by Low-Coherent Full-Field Quantitative Phase Microscopy

Toyohiko Yamauchi
Hamamatsu Photonics

Wednesday, April 28, 2010 at 2:00pm
Engr IV Maxwell Room 57-124

Abstract
We recently developed low-coherent full-field quantitative phase microscopy for obtaining images of cell membrane morphology. Our setup is based on a Linnik-type phase-shifting interferometric microscope with a coherence length of 1.5 m. The optical wavefront of the light reflected from the cell membrane is imaged and provides the three-dimensional topography of a cell membrane without a priori assumption of the cell's refractive index. In addition, stabilization of the optical path difference between the sample and the reference arm is introduced to realize 1.2-nanometer long-term stability of the height measurement over 500 seconds. In this presentation we report our analysis of membrane fluctuations of different types of cells and of cells under different conditions in a recording that lasts several minutes. We believe that these results imply the feasibility of the low-coherent quantitative phase microscope for cell classification without fluorescent labeling.

[1] Toyohiko Yamauchi, Hidenao Iwai, Mitsuharu Miwa, and Yutaka Yamashita, "Low-coherent quantitative phase microscope for nanometer-scale measurement of living cells morphology," Opt. Express 16, 12227-12238 (2008) [2] Toyohiko Yamauchi, Norikazu Sugiyama, Takashi Sakurai, Hidenao Iwai, and Yutaka Yamashita, "Label-free classification of cell types by imaging of cell membrane fluctuations using low-coherent full-field quantitative phase microscopy," Proc. SPIE 7570, 75700X (2010), DOI:10.1117/12.841008

Biography
Mr. Yamauchi graduated with Bachelor's and Master's degrees in Engineering from the University of Tokyo in 2002 and 2004, respectively. He has been working at Hamamatsu Photonics K.K. (Japan) since 2004 as a researcher in the central research group. He has also been a visiting researcher for a collaborative study at the MIT Spectroscopy Laboratory since 2008. He is engaged in research on biomedical sensing with interference optics.