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Lensfree Fluorescent Computational Microscopy on a Chip

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What
  • PhD Defenses
When Apr 15, 2013
from 02:00 PM to 05:00 PM
Where Engr. IV Bldg., Tesla Room 53-125
Contact Name Ahmet Coskun
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Ahmet F. Coskun

Advisor: Prof. Aydogan Ozcan

Abstract

Optical Microscopy has become an indispensible tool for many scientific disciplines especially in biomedical sciences. Although rapid advancements in modern microscopy techniques allow us to visualize the microscopic structures and processes in unprecedented details, they are still relatively bulky and low-throughput, necessitating a tedious mechanical scanning to image large-area micro-systems. In this dissertation, I demonstrate an on-chip computational microscopy platform as an alternative high-throughput screening tool that can rapidly monitor fluorescently labeled cells or small animal models over an ultra-wide field-of-view (FOV) of e.g., >9-18 cm2 without the use of any lenses, thin-film filters or mechanical scanners. Probing a large volume (e.g., >0.1 mL) of sample through the use of an excitation interface (e.g., prism, hemisphere or waveguide) and incoherent sources such as light emitting diodes (LEDs), in this platform only the fluorescent emission is collected via fiber-optic arrays and delivered to a large-format opto-electronic sensor array (e.g., CCD or CMOS). Acquired raw fluorescent images are then decoded based on a compressive sampling algorithm, yielding higher resolution microscopic images. The presented on-chip imaging approach, being a compact fluorescent encoder, together with a compressive decoder behind it, achieves < 3-4 μm spatial resolution with multi-color and multi-layer imaging capabilities on the same platform. Such a computational fluorescent microscopy technique could pave the way toward rapid diagnostic systems for biomedical applications, including on-chip cytometry, rare-cell analysis, as well as small animal research.

Biography

Ahmet F. Coskun received his BS degrees in Electrical Engineering and Physics from Koc University, Turkey and MS degrees in Electrical Engineering (Major) and Chemistry and Biochemistry (Minor) from University of California, Los Angeles (UCLA). He is now pursuing his PhD degree in Electrical Engineering at UCLA, where he is conducting research in the Biophotonics Lab at UCLA under the supervision of Prof. Aydogan Ozcan. He has authored or co-authored 2 book chapters, and more than 50 peer reviewed research articles in major journals and conferences.

He is also a member of the Optical Society (OSA); the International Society for Optics and Photonics (SPIE); the Institute of Electrical and Electronics Engineering (IEEE), and the Biomedical Engineering Society (BMES). He is also the founding president of the SPIE Student Chapter and the founding vice president of the OSA Student Chapter at UCLA.

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