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Lensfree Optical Tomography for High-Throughput 3D Imaging on a Chip

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What
  • PhD Defenses
When Oct 25, 2012
from 03:00 PM to 05:00 PM
Where Engr. IV Bldg. Tesla Room 53-125
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Serhan O. Isikman

Advisor: Associate Professor Aydogan Ozcan

 

Abstract

Recently, optical microscopy has seen a growing interest in developing three-dimensional (3D) imaging techniques that enable sectional imaging of biological specimen. Existing 3D imaging techniques, however, are generally quite complex, bulky and expensive in addition to having a limited field-of-view due to the need for lens-based optical magnification.

In this dissertation, I demonstrate lensfree optical tomography (LOT) as a new 3D imaging modality that offers high-throughput imaging in a compact and simple architecture. This technique is based on multi-angle lensfree on-chip microscopy, where computation is used to replace bulky components of traditional imaging devices to reduce size, cost and complexity while at the same time significantly enlarging the imaging field-of-view. LOT offers <350 nm lateral resolution and ~2 mm axial resolution over large imaging volumes of e.g., 5-100 mm3, and can be assembled in a lightweight and compact architecture.

To demonstrate that LOT could be useful for imaging applications in resource-limited settings, I also devised a field-portable, compact and lightweight tomographic microscope that only weighs ~110 grams. This portable device can fit in a volume of 96 mm x 89 mm x 40 mm. In addition, to demonstrate the integration of LOT in microfluidic platforms, I also introduce an optofluidic tomography platform, where the sample is delivered to the tomographic imager through a microfluidic chamber, which is mounted on the sensor chip. Tomographic data acquisition is performed while the objects are electrokinetically driven through the micro-chamber.

Probing a large volume at micrometer-scale 3D spatial resolution, LOT could provide a powerful imaging tool for high-throughput imaging applications in e.g., cell and developmental biology, as well as for future lab-on-chip platforms.

 

Biography

Serhan O. Isikman is a PhD candidate at the department of Electrical Engineering at UCLA.  He earned the B.S. and M.S. degrees from Koc University in Turkey, both in Electrical Engineering. His research in optical microsystems and biophotonics has led to 2 book chapters, and more than 50 journal and conference publications.

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