Intra-molecule Motion of Functional Proteins at Single Molecule Level Using Synchrotron X-ray

Speaker: Hiroshi Sekiguchi, Ph.D.
Affiliation: Japan Synchrotron Radiation Research Institute

Abstract:

We have proposed a single molecule technique that utilizes short wavelength probes of X-rays to monitor the internal motions of a single protein. We call it diffracted X-ray tracking (DXT)[1,2] and it would be a powerful technique in biological science for detecting atomic-scale dynamic motion of the protein at the single molecular level at several tens of microseconds time resolution. In DXT, a target protein is labeled with a nanocrystal with a size of several tens of nanometers and the motions of the nanocrystal coupled with the protein’s motions are recorded as the trajectories of the Laue spots from the nanocrystal.

At the seminar, we will present recent DXT results for dynamic motion of multimeric proteins [3-6] and discuss the method in the future. And we also want to discuss the X-ray fluorescence holography technique which we start from last year for visualizing local structure of proteins (http://www.en.3d-activesite.jp/).

For more information, contact Prof. Aydogan Ozcan (ozcan@g.ucla.edu)

Date/Time:
Date(s) - Mar 04, 2016
1:00 pm - 2:00 pm

Location:
E-IV Tesla Room #53-125
420 Westwood Plaza - 5th Flr., Los Angeles CA 90095