Single pixel high-throughput microscopy on a tight budget: signal reduction from ultrafast optical-time stretch imaging to ultraslow 3D holography

Speaker: Antony C. Chan
Affiliation: Ph.D. Student, University of Hong Kong

Abstract: Optical time-stretch microscopy is an unconventional technique for capturing non-recurring events at 10MHz line scan rate — a rate unprecedented by imaging sensor arrays such as CMOS or CCD. Optical scanning holography records volumetric information of fluorescence objects with single-pixel photomultiplier at single-photon sensitivity — again unmatched by imaging sensor arrays. Both technologies have attracted interest in applications from high-throughput cellular imaging flow cytometry to in-vivo fluorescence volumetric microscopy. Although they lie on the opposite end of the imaging speed spectrum, they both share a common drawback in high-throughput microscopy. In fact, both techniques generate gigabytes of pixels in the acquisition process that entails a stringent requirement of the state-of-the-art hardware in digitization, storage, and image reconstruction at the total cost of up to $0.1M. However, all these stringent requirements can be mitigated by harnessing a tight integration of illumination optics and the power of computational imaging. In my talk, I will present the design process involved to enable computational image restoration at lower equipment specifications, e.g. numerical aperture, sampling rate and memory buffer. Eventually, this will lower the barriers to entry for adoption of the high-throughput microscopy technology.

Biography: Antony C. Chan received the B. Eng. (1st class honor) degree in Medical Engineering from the University of Hong Kong in 2012. He is currently a Ph.D. candidate in the Department of Electrical and Electronic Engineering at the University of Hong Kong. His research interests include ultrafast real-time cellular microscopy and computational image restoration for biomedical application. He was selected to participate at the Siegman International School on Lasers in 2014, and was awarded an honorable mention in the poster sharing session. He also received the Newport Spectra-Physics Research Excellence Travel Award for the Photonics West Conference in 2016.

For more information, contact Prof. Aydogan Ozcan ()

Date(s) - Feb 26, 2016
3:00 pm - 4:00 pm

E-IV Maxwell Room #57-124
420 Westwood Plaza - 5th Flr. , Los Angeles CA 90095